Expression of the major structural protein(VP2-VP3)gene of a Chinese isolate,goose parvovirus in E.coli

According to nucleotide suquence of GPV A strain,a pairs of primers were designed and synthesized,and mutated the VP2ACG codon to ATG one.The complete VP2-VP3(the major structural protein)genes of a Chinese isolate stain(GPV CC)was amplified by PCR,and obtained a DNA fragment with the sizes of1.8kb.The PCR prod-uct was digested with Bam HI and the resulting fragment was inserted into the Bam HI site of the pET28(a)under the downstream sequences.E.coli component host BL21was transformed with the recombinant.The recombinant bac-teria could express the VP2-VP3gene after induced by IPTG,and the recombinant protein were analyzed on12%SDS-PAGE and stained with comass brilliant blue or had been detected by immunblotting analysis with anti-GPV serum.