Study on the Extraction Process of Total Flavonoids from Smilax Glabra by Enzyme-assisted Hydrolysis

The total flavonoids was Extracted from Smilax glabra by a enzyme-assisted hydrothermal method;Using Rutin as standard,the content of the total flavonoids was measured by ultraviolet and visible spectrophotometry with aluminum ion colour.The extraction ratio of total flavonoids was investigated as a main target.The effects of amount of enzyme,the solid-liquid ratio,extraction temperature and extraction times on the total extraction ratio of flavonoids were discussed by means of the single-factor experiment to optimize the extraction conditions.The results showed that: for the best extraction conditions the enzyme dosage is 2.0% with the solid-liquid ratio being 1:8 and extraction frequency being 3 times at 100 ℃.In the best conditions,the powder ratio of crude extraction is 14.0%,and the content of total flavonoids in this powder is up to 4.1% with the extract ratio of total flavonoids being 0.57%.