Study on Extraction Process of Total Flavonoids from Smilax Glabra by Enzyme-assisted Hydrolysis

The total flavonoids were extracted from smilax glabra with enzyme-assisted hydrothermal method.The content of the total flavonoids was measured by UV-vis spectrophotometry under assisited with aluminum ion colour using Rutin as standard.Extraction ratio of total flavonoids was investigated as a main target.The effects of enzyme added volume,enzyme hydrolysis time and media pH value on the extraction rate were discussed.The results showed that the optimum extract conditions are as follow:when enzyme concentration is 1.0%,hydrolysis time is 3.0 h,enzymatic hydrolysis media pH value is 5.0,the powder ratio of crude extraction is 13.9%,and the content of total flavonoids in this powder is up to 4.0 % with the extract ratio of total flavonoids being 0.53%.