Determination of chlorogenic acid and baicalin in Mumps Mixture by HPLC

Objective To determine the contents of chlorogenic acid and baicalin in Mumps Mixture by HPLC.Methods Hypersil ODS C18 column(150 mm×2.5 mm,5 μm) was used.The mobile phase was methanol-0.4% phosphoric acid(gradient elution).The flow rate was 0.8 ml·min-1,and the detecting wavelength was at 327 nm.Results The linearies of chlorogenic acid and baicalin were 6.88~68.80 mg·L-1(r=0.999 9,n=7) and 13.95~139.50 mg·L-1(r=0.999 9,n=7).The average recoveries were 101.08% and 100.39%,respectively.The RSDs were 0.89%(n=5) and 0.66%(n=5),respectively.Conclusion This method is simple,accurate and repeatable.It can be used for quality control in this preparation.