The Expression of TLR2 mRNA in Rat Peritoneal Mesothelial Cells Stimulated by Lipopolysaccharide,Rosiglitazone and Highglucose

Objective To observe the effect of rosiglitazone on the mRNA expression of toll-like receptor 2 in rat peritoneal mesothelial cell (RPMC) stimulated by Lipopolysaccharide(LPS) and to observe the expression of toll-like receptor 2 in RPMC stimulated by highglucose. Methods RPMC were isolated from rat colic omentum and were incubated with LPS (1.0 μg/ml,10 μg/ml,100 μg/ml),or stimulated by Rosiglitazone(10 μmol/L) after incubated with LPS (10 μg/ml) for 2 h. The RPMC were under the stimulation of highglucose (1.5%,2.5%,4.25%). RPMC in the control group were just incubated with medium. TLR2 mRNA was detected by RT-PCR. Results Compared with the control group,the expression of TLR2 mRNA was significantly increased in the groups stimulated by LPS and highglucose. In the group pre-incubated with LPS,compared with the group stimulated by LPS,the up-regulating expression of TLR2 mRNA was significantly inhibited. Conclusion LPS and highglucose can up-regulate the expression of TLR2 mRNA in rat peritoneal mesothelial cell. Rosiglitazone can inhibit the up-regulating effect of LPS on the expression of TLR2 mRNA.