[Expression of IL-18, IL-6 and oxidative stress in rat peritoneal mesothelial cells stimulated by lipopolysaccharide].

AIM: To observe the expression of IL-18, IL-6 and the production of oxidative stress Malondialdehyde (MDA) in rat peritoneal mesothelial cells (RPMC) stimulated by lipopolysaccharide (LPS). METHODS: RPMC were primordially culture and stimulated with different concentrations (1, 10, 100 mg/L) LPS for 6h; RPMC were stimulated by 10 mg/L LPS for 3, 6, 12, 24 h. IL-18 mRNA was detected by real time-PCR. IL-6 and IL-18 were detected by ELISA in supernatants. MDA was measured by thiobarbiuric acid method. RESULTS: Compared with the control group, the expression of IL-18, IL-6 and MDA were gradually increased by LPS with different concentrations (P < 0.05) ; the expression of targets above were little by little increased with stimulated time prolonging. then the peak of IL-18 were appeared in 12 h. CONCLUSION: The expression of proinflammatory cytokine IL-6 and the production of oxidative stress MDA was increased in the RPMCs stimulated by LPS, then leads to the perpetuating amplificated inflammation, aggravate peritoneal impairment and hyperfiltration losed.