External use of mitomycin C can inhibit the hypertrophic scarring of rabbit ears

Objective To observe the inhibitory effect of different concentrations of mitomycin C on rabbit ear hypertrophic scar. Methods Rabbit ear hypertrophic scars model was established in 18 Japanese white rabbits. The animals were randomly divided into four groups: normal saline group( A),0. 1 mg / ml mitomycin C group( group B),0. 05 mg / ml mitomycin C( group C) and 1 mg / ml triamcinolone group( group D). The fresh wounds of the animals were externally given and kept with the corresponding treated solutions in each group. Then,the thickness of hypertrophic scar after wound healing was counted with a color Doppler. Further,the scar tissues were removed for HE staining,and fibroblast cells under a single vision were counted with a microscopy( × 200 times). Results The scar thickness and fibroblast cells in the group A were significantly greater than that in the groups B,C or D( P 0. 05). The scar thickness and fibroblast cells in the group C were also significantly greater than that in the group B( P 0. 05). There was no significant difference between the group B and group D. No incision necrosis and delayed healing was found in the group B and C. Conclusion External application of mitomycin C can inhibit the growth of fibroblast cells and scar hyperplasia in rabbit ears and does not induce the local tissue necrosis. Thus,it has a relatively high safety.