ScholarLens Back to search
2007•Journal of Tianjin Agricultural UniversityRequires access

Callus Formation and Bud Regeneration of Melilotus suaveolens

Lei Zhang

Open publisher page 0 citations

Abstract

In order to set up the effective regeneration system and gene transformation system,the callus formation and bud regeneration of Melilotus suaveolens were researched with MS,1/2 MS and different plant hormones.The results show that the ratio of callus formation was the highest in the culture medium of MS + 2,4-D 1.0 mg.L-1 + KT 0.3 mg.L-1,which was 90%.The bud regeneration was the best in the culture medium of MS + 2,4-D 0.2 mg.L-1 + 6-BA 1.0～1.5 mg.L-1,which was 45%.And,the rootability was 100% when the regeneration buds grew on the culture medium of 1/2 MS + IAA 0.5 mg.L-1.

About this research paper

What this paper is about

In order to set up the effective regeneration system and gene transformation system,the callus formation and bud regeneration of Melilotus suaveolens were researched with MS,1/2 MS and different plant hormones.The results show that the ratio of callus formation was the highest in the culture medium of MS + 2,4-D 1.0 mg.L-1 + KT 0.3 mg.L-1,which was 90%.The bud regeneration was the best in the culture medium of MS + 2,4-D 0.2 mg.L-1 + 6-BA 1.0～1.5 mg.L-1,which was 45%.And,the rootability was 100% when the regeneration buds grew on the culture medium of 1/2 MS + IAA 0.5 mg.L-1.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

In order to set up the effective regeneration system and gene transformation system,the callus formation and bud regeneration of Melilotus suaveolens were researched with MS,1/2 MS and different plant hormones.The results show that the ratio of callus formation was the highest in the culture medium of MS + 2,4-D 1.0 mg.L-1 + KT 0.3 mg.L-1,which was 90%.The bud regeneration was the best in the culture medium of MS + 2,4-D 0.2 mg.L-1 + 6-BA 1.0～1.5 mg.L-1,which was 45%.And,the rootability was 100% when the regeneration buds grew on the culture medium of 1/2 MS + IAA 0.5 mg.L-1.

Key concepts: Callus, Melilotus, Regeneration (biology), Botany, Biology, Tissue culture, Transformation (genetics), Murashige and Skoog medium

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Callus Formation and Bud Regeneration of Melilotus suaveolens — Research Paper | ScholarLens