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2013•China Animal Husbandry & Veterinary MedicineRequires access

Study on Proliferation Characteristics and Pathogenicity of Pseudorabies Virus Ra Strain in Different Cells

YU Wen-la

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Abstract

To select the most suitable cell line for research on the pathogenic mechanism and vaccine of pseudorabies virus(PRV)Ra strain,different kinds of cells(ST,PK-15,Vero,F81,DF1,MDCK and CEF)were chosen to be inoculated with PRV.Cytopathy effect(CPE)characteristics,time and titers of the cell cultures were compared in this study.According to the morphology observation of infected cells,we found that the PRV Ra strain could proliferate in these seven kinds of cells and cause significant CPE,and the CPE obviously appeared firstly within 24hon the PK-15 and ST cells.The TCID50 of PRV in the seven kinds of cell cultures performed differently,and the TCID50 of infected ST cell was the highest.Therefore,it suggested that ST cell could be used in the further study of PRV Ra strain in vitro.

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What this paper is about

To select the most suitable cell line for research on the pathogenic mechanism and vaccine of pseudorabies virus(PRV)Ra strain,different kinds of cells(ST,PK-15,Vero,F81,DF1,MDCK and CEF)were chosen to be inoculated with PRV.Cytopathy effect(CPE)characteristics,time and titers of the cell cultures were compared in this study.According to the morphology observation of infected cells,we found that the PRV Ra strain could proliferate in these seven kinds of cells and cause significant CPE,and the CPE obviously appeared firstly within 24hon the PK-15 and ST cells.The TCID50 of PRV in the seven kinds of cell cultures performed differently,and the TCID50 of infected ST cell was the highest.Therefore,it suggested that ST cell could be used in the further study of PRV Ra strain in vitro.

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Available abstract

To select the most suitable cell line for research on the pathogenic mechanism and vaccine of pseudorabies virus(PRV)Ra strain,different kinds of cells(ST,PK-15,Vero,F81,DF1,MDCK and CEF)were chosen to be inoculated with PRV.Cytopathy effect(CPE)characteristics,time and titers of the cell cultures were compared in this study.According to the morphology observation of infected cells,we found that the PRV Ra strain could proliferate in these seven kinds of cells and cause significant CPE,and the CPE obviously appeared firstly within 24hon the PK-15 and ST cells.The TCID50 of PRV in the seven kinds of cell cultures performed differently,and the TCID50 of infected ST cell was the highest.Therefore,it suggested that ST cell could be used in the further study of PRV Ra strain in vitro.

Key concepts: Pseudorabies, Vero cell, Strain (injury), Virus, Virology, Titer, Cell culture, Biology

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