Expression of Nanog in non-small cell lung cancer tissue

Aim:To investigate the expression of Nanog in non-small cell lung cancer( NSCLC) tissue. Methods:The expressions of Nanog mRNA and protein were detected in the specimens from 106 cases of NSCLC and 10 cases of normal lung tissue by using quantitative real-time PCR and immunohistochemistry,respectively,then the relationship between the expression of Nanog protein and the prognosis of the patients with lung adenocarcinoma( LAC) or lung squmous cell carcinoma( LSCC) was analyzed by log-rank test. Results:The positive expression rate of Nanog protein in NSCLC tissue was24. 5%,higher than that( 0%) in normal lung tissue( P = 0. 114); the positive expression rate of Nanog protein was higher in LAC tissue than that in LSCC tissue and increased with the increase of pathological degree( χ2= 5. 087,6. 845,P 0. 05); in patients with LAC,those with negative expression of Nanog protein had better progonosis( χ2= 5. 352,P =0. 020). The expression level of Nanog mRNA in NSCLC tissue was( 0. 950 ± 0. 183),higher than that( 0. 277 ± 0. 190)in normal lung tissue( F = 122. 662,P 0. 001); the expression level of Nanog mRNA increased with the increase of pathological degree( F = 4. 047,P = 0. 020). Conclusion:Nanog expression is relevant to the carcinogenesis and development of NSCLC,besides,the expression of Nanog protein is associated with the prognosis of patients with LAC.