Study on the Dissolution Characteristics and Speciation of Trace Elements in Amomum villosum by ICP-MS

Objective To analyze the dissolution characteristics and speciation of trace elements in Amomum villosum and investigate the bioactivity of trace elements.Methods The content and dissolved rate of 13 trace elements in Amomum villosum were analyzed by ICP-MS with 0.45 μm membrane,the n-C8H17OH-water extraction system respectively.Divided the decoction of Amomum villosum into several species,including the soluble species,suspended species or the n-C8H17OH-soluble species and water-soluble species,and studied every species.Results The correlative coefficients of the analytical calibration curves were 0.999 8~1.000 0,and RSD was 0.1%~5.0%.The accuracy and precision of the method were tested by comparing the values obtained from the determination of the standard sample,bush twigs and leaves(GBW07603)by this method with the reference values of GBW07603.The determination results were found to be basically consistent with the reference values.The highest dissolved rate was Co in the decoction of Amomum villosum,the dissolved rate of element did not depend on the content of trace element in the drug.The trace elements of Amomum villosum mainly existed in suspended species.Only Cr,its content in soluble species was more than 50% of its whole content in all species,and the Kow values of Fe,Mo,and Cd were all more than 1 under the pH of intestinal and stomach juice,while Cr,Ni were less than 1.Kow values of trace elements was influenced by different pH values.Conclusion The dissolution characteristics and speciation of trace elements in Amomum villosum,is related with the bioactivity and efficacy of Amomum villosum to some extent.