Study on the Extraction,Purification and Structural Characterization of Polysaccharide from Enteromorpha

Polysaccharide was extracted from Enteromorpha by hot water-papain.The crude Enteromorpha polysaccharide was separated and purified by DEAE ion-exchange cellulose chromatography and four kinds of polysaccharide components were obtained.The identification of purification with Sephadex G-100 gel chromatography implied that EP1 was homogeneous polysaccharide.From the result of FTIR,EP1 had the polysaccharide characteristic absorption peak and the sulfur acid radical existence.There were no nucleic acid and protein absorbance at 260 nm and 280 nm on the ultraviolet spectrum.The monosaccharide composition of EP1was analyzed by HPLC,which was Man ∶ Rha ∶ Glu ∶ Xyl = 0.09 ∶ 1.0 ∶ 3.1 ∶ 0.29。