Purification and Spectroscopic Analysis of Polysaccharide from Mango

Crude polysaccharide was extracted from Mexico mango using hot water extraction followed by ethanol precipitation.The polysaccharide concentration of crude extract was(46.3 ± 1.75) mg/g as determined by phenol-sulfuric acid assay.After further purification by DEAE-52 and Sephadex G-75 column chromatography,a white flocculent polysaccharide was obtained.High performance anion exchange chromatography with pulsed amperometric detection(HPAEC-PAD) was used to determine the monosaccharide composition.The results showed that the polysaccharide was composed of rhamnose,galactose and glucose with a ratio of(6.3±1.40),(11.9 ±1.76) mg/g and(13.8 ±1.52) mg/g,respectively.The purified polysaccharide exhibited the characteristic IR absorption of polysaccharide.The structure was pyranoid glucan with β-glycosidic bond.