Separation,Purification and Structural Analysis of Polysaccharide from Chinese Pine Pollen

A polysaccharide from Chinese pine pollen was obtained through hot water extraction,ethanol precipitation,Sevag deproteinization and Sephadex G-100 chromatography and was analyzed structurally by IR spectroscopy,UV-vis spectroscopy and gas chromatography (GC). The polysaccharide was highly heterogeneous and was mainly composed of rhamnose arabinose,xylose,mannose,glucose and galactose. Besides,GC analysis revealed that the polysaccharide contained an unknown monosaccharide with a small peak area suggesting low content. No characteristic absorption of nucleic acid or protein in the polysaccharide was observed in its UV-vis spectrum and it exhibited characteristic IR absorption of polysaccharide.