Influence of Jianpi Huoxue Decoction on Pathological Changes and Expression of Hepatic Collagen Fibers in Rats with Liver Fibrosis

Objective: To investigate the effect of Jianpi Huoxue Decoction on liver function,serum fibrosis indices,liver pathological changes and the expression of hepatic collagen fibers in rats with liver fibrosis.Methods: Hepatic fibrosis was induced by carbon tetrachloride hypodermic injection.The Wistar rats were randomly divided into 5 groups: normal group,model control group,colchicines group,high-dose and low-dose groups of Jianpi Huoxue Decoction.All medicine was given by intragastric administration.At the end of treatment,serum alanine amino transamine(ALT),aspartate transamine(AST),albumin(Alb) and total bilirubin(TBil),serum hyaluronic acid,laminin and precollagen Ⅲ were detected.HE and Masson staining of liver tissue were determined.Results: Jianpi Huoxue Decoction can obviously decrease serum levels of ALT,AST,TBIL,HA,LN and PCШ,improve the level of ALB of rats;HE and Masson staining showed that Jianpi Huoxue Decoction can obviously reduce the expression of collagen fibers of liver tissue in hepatic fibrosis rats.Conclusion: Jianpi Huoxue Decoction can effectively prevent the pathological process in hepatic fibrosis rats.The mechanisms might be related with the protective effects on hepatic cells and the inhibition on collagen fibers synthesis.