The epidemiology trend and the laboratory test of hand,foot and mouth disease in Suzhou city in 2010

Objective: To analyze pathogen surveillance data of hand,foot and mouth disease(HFMD) in Suzhou in 2010,to provide scicentific references for prevention and contrl policy and to study the application value of fluorescence real-time polymerase chain reaction(PCR) in detecting nucleic acid of the virus causing HFMD.Methods: 1711 throat swabs were collected from patients who were clinical diagnosed as HFMD,nucleic acid were purified and identified by fluorescence real-tim PCR,then EV71 and CoxA16 RNA was detected in the total enterovirus positive samples.Results: HFMD could happen in all four seasons,but most cases occurred from April to July.Children aged 1～4 years old were high risk group,and infected boys are much more than that of girls.EV71 accounted for most of the cases of serious conditions.Conclusion: EV71 was dominant virus in HFMD in Suzhou city in 2010.Fluorescence real-time PCR can be used as a rapid diagnostic method because of its sensitivity,specificity and efficiency,which are very important in prevention and control of enterovirus infection.