Analysis of pathogenic detection of hand-foot-mouth disease cases in Hainan Province in 2009

Objective To investigate the main virus type which caused pandemic of hand-foot-mouth disease(HFMD) in Hainan province in 2009.Methods According to the Ministry of Health issuedHand-foot-mouth disease control and prevention guide(2009) nucleic acids from HFMD specimens,such as nasal and throat swabs,anus swabs,herpes juice and so on extracted for clinical diagnosis of HFMD.The RNA was amplified by reverse transcription-polymerase chain reaction(RT-PCR) and real-time fluorescence PCR using the enterovirus universal primer,enterovirus type 71 primer and Coxsackie virus group A type 16 primer,as well as detecting products size.The nucleic acids positive specimens were inoculated to RD cell lines to isolate enteroviruses.Results The RNA positive rates of EV71,CoxA16 and other type EV were respectively 23.13%,1.57% and 9.59% in 1 085 specimens of HFMD in 2009.The positive rates of death cases,severe cases and ordinary cases were 55.56%(10/18),36.07%(145/402)and 32.63%(217/665)respectively.The positive rate of different specimens was different.The positive rate of anus swabs and feces was 49.62%.The rate of herpes juice was 43.84%.The positive rate of nasal and throat swabs was 22.89%.Conclusion The major pathogen of HFMD was EV71 and CoxA16 was also one of the pathogen.The pathogenic monitoring of HFMD should be strengthened in order to know the change of virus type and provide technical support for HFMD prevention and control.