Study on the Mechanism of Baicalin in Psoriasis Treatment

Objectives To study the mechanisms of baicalin in psoriasis treatment. Methods Human benign epidermal keratinocytes (HaCaT cells), human fibroblasts were treated with different concentration of baicalin in vitro. The growth inhibition effects were determined by measuring MTT absorbance of living cells, and cell cycle distribution was detected by flow cytometry. Results Baicalin in concentration from 1.5 μg/mL to 96 μg/mL inhibited the proliferation of fibroblasts in a dose and time dependent manner, but it had no obvious effects on keratinocytes and peripheral blood mononuclear cells (PBMCs). Further study by DNA flow cytometric analysis revealed that cell percentage of fibroblasts, rather than HaCaT cells, in G0 to G1 phase increased and those in G2M, S phase decreased with the denser of baicalin' s concentration. Conclusion It suggested that baicalin could inhibit the proliferation of fibroblasts, further to affect the proliferation of keratinocytes, which was one of its mechanisms of psoriasis treatment.