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Callus and Shoots Induction of Gerbera jamesoni

Wenjing Hu

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Abstract

The stem nodes with buds of Gerbera jamesoni were used as explants,the effects of different hormone combinations on callus and shoots induction were studied.The results showed that the explants were treated by 0.1% HgCl2 for 10 min.The optimum medium for callus induction was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L,and MS+6-BA 2.5 mg/L+NAA 0.05 mg/L was suitable for shoots induction.The shoots was multiplied and induced to form roots,and finally vigorous plantlets with dark green color were regenerated.

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What this paper is about

The stem nodes with buds of Gerbera jamesoni were used as explants,the effects of different hormone combinations on callus and shoots induction were studied.The results showed that the explants were treated by 0.1% HgCl2 for 10 min.The optimum medium for callus induction was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L,and MS+6-BA 2.5 mg/L+NAA 0.05 mg/L was suitable for shoots induction.The shoots was multiplied and induced to form roots,and finally vigorous plantlets with dark green color were regenerated.

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Available abstract

The stem nodes with buds of Gerbera jamesoni were used as explants,the effects of different hormone combinations on callus and shoots induction were studied.The results showed that the explants were treated by 0.1% HgCl2 for 10 min.The optimum medium for callus induction was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L,and MS+6-BA 2.5 mg/L+NAA 0.05 mg/L was suitable for shoots induction.The shoots was multiplied and induced to form roots,and finally vigorous plantlets with dark green color were regenerated.

Key concepts: Callus, Gerbera, Shoot, Explant culture, Botany, Murashige and Skoog medium, Biology, Chemistry

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