Chemical constituents in stems with hooks of Uncaria sessilifructus

Objective To study the chemical constituents from the stems with hooks of Uncaria sessilifructus.Methods The chemical constituents were isolated and purified by chromatography on silica gel,MCI,Sephadex LH-20 columns,etc.The structures of compounds were elucidated by spectral analyses.Results Fourteen non-alkaloids compounds were isolated from the 95% ethanol extract from the stems with hooks of U.sessilifructus,and the structures were identified as friedelin(1),betulin(2),obtusalin(3),stigmast-4-en-3-one(4),β-sitosterol(5),β-daucosterol(6),24-en-cycloartenone(7),squalene(8),vitamin E(9),α-tocopherolquinone(10),(-)-alloaromadendrene(11),eugenin(12),scopoletin(13),and bis(2-ethylhexyl) phthalate(14); Three alkaloids were identified as mitraphylline(15),isomitraphylline(16),and isomitraphylline N-oxide(17).Conclusion compounds 1,4,8,11,and 13 are obtained from the plants of genus Uncaria Schreber nom.cons.for the first time,and all the above compounds are obtained from the stems with hooks of U.sessilifructus for the first time.