Significance of combined determination of anti-SmD1, anti-nucleosome and anti-double stranded DNA in the diagnosis of systemic lupus erythematosus

Objective To evaluate the significance of combined determination of anti-SmD1, anti-nucleosome (AnuA) and anti-double stranded DNA (dsDNA) in diagnosing systemic lupus erythematosus (SLE). Methods In 40 patients with SLE, 14 patients of other autoimmune diseaes and 20 healthy people, serum anti-dsDNA antibody was determined by radioimmunoassay, anti-SmD_1 and AnuA were tested by IMTEC line immuno assay. Results The positive rate of anti-dsDNA, anti-SmD1 and AnuA in the patients with SLE was 42.5%, 80.0% and 72.5% respectively. After combined determination of anti-dsDNA, anti-SmD1 and AnuA, the positive rate rose to 87.5%, and the diagnosis efficiency was 85.2%. Conclusions The combined determination of anti-dsDNA, anti-SmD1 and AnuA may improve the sensitivity and efficiency of laboratory diagnosis of SLE and has an important significance in diagnosis and treatment of SLE.