The influence of sulfur fumigation on the content of ferulic acid in Radix angelicae sinensis

Objective To explore the influence of sulfur fumigation on ferulic acid in Radix angelicae sinensis.Methods The content change of ferulic acid after sulfur fumigation was determined by HPLC on XB-C18 column(4.6 mm×250 mm,5 μm).The mobile phase was acetonitrile-0.085% phosphoric acid solution(17:83).The detection wavelength was 316 nm,the column temperature was 35 ℃ and the flow rate was 1.0 mL·min-1.Results The maximum content of ferulic acid was obtained through fumigation with 100 g·cm-3 sulfur once for 2 h;The linear relationship of ferulic acid was good in 32.32-323.20 μg,and the regression equation was Y=53 835X-57 496(r=0.999 8).Conclusion Sulfur fumigation had certain influence on the content of ferulic acid in Radix angelicae sinensis.The content of ferulic acid showed a decreasing trends with increasing of amount of sulfur,fumigation time and the number of fumigation.