Effects of Arsenic on Proliferation and Differentiation of 3T3-L1 Preadipocytes

To observe the effect of arsenic on the proliferation and differentiation of 3T3-L1 preadipocytes.The differentiation of 3T3-L1 preadipocytes was identified with red O staining,the proliferation of 3T3-L1 preadipocytes was detected by MTT and cell calculation.Lipid droplets accumulated in cytoplasm of the differentiated preadipocytes were observed by using red O staining and quantified by colorimetry.The results showed that the low concentration of arsenic(low than 5μmol/L) affected 3T3-L1 preadipocytes proliferation was significantly increased,but in contrast arsenic at different concentrations(5,10,15,20 and 25μmol/L) which affected cell proliferation was decreased(P0.05,compared with the normal control group).Whatever arsenic at any concentration was significantly decreased the differentiation of adipocytes and does-dependently.Arsenic at low concentration can increase the proliferation of 3T3-L1 preadipocytes,and arsenic at high or low concentration inhibit the differentiation of adipocytes.