Effect of selenium on proliferation and differentiation of 3T3-L1 cells

Objective: To study the effect of selenium on proliferation and differentiation of 3T3-L1 preadipocyte.Methods: The 3T3-L1 preadipocytes were cultured and divided into control group,low selenium group,middle selenium group,and high selenium group(The concentrations of sodium selenite were 0μmol/L,0.01 μmol/L,0.1 μmol/L,1 μmol/L),the effect of selenium on the proliferation of 3T3-L1 was used to determine by MTT.Oil red O staining was used to determine the differentiation rate and level of 3T3-L1 preadipocytes.Results: Of the proliferation,compared with the control group,OD value all decreased in the trial groups,and had dose-dependent(P0.05);of the differentiation,compared with the control group,OD value had no markedly change,there was no significant difference(P0.05).Conclusion: Selenium does not affect the differentiation of preadipocytes,but inhibit the proliferation of preadipocytes.