Development of AFLP Molecular Markers Tightly Linked with Ps-2 Loci in Tomato

Tomato germplam with ps-2 gene could easily produce the progeny with 100% sterility through selfing.It has been conveniently used for F_1 hybrid production.In this paper,123 individuals of F_2 segregated population were derived from Bulgaria line CMC1ps2 containing ps-2 gene as a male parent and a virtual processing tomato line 92155 as a female parent.The segregation ration of fertility and sterility in this population completely meet the Madeline genetic mechanism as a single recessive gene.Sixty-four AFLP primer combinations were screened and 3 AFLP markers including E37/M47、E38/M48 and E33/M50 had been identified to tightly linked with Ps-2 fertile loci.The genetic distances were respectively 6.04cM、6.04cM、6.06cM,and all of them were located one side with Ps-2 loci.These markers amplified respectively 390bp、150bp、80bp DNA fragment.Three AFLP markers obtained in this paper could be directly used for maker-assistant selection and speed up the introgression and use of male sterility for tomato production.