Development of SSR and CAPS Molecular Markers Linked with ps-2 Gene in Tomato

Tomatoes with ps-2 gene can produce the progeny with 100% male sterility through selfing hence it is easy to be used for hybrid production.An elite processing tomato inbred line 92155 as male parent and a line named as CMC1ps2 with ps-2 gene from Bulgaria as female parent were employed to produce a F2 segregation population with 123 individuals.The segregation ratio of sterility was consistent with the Mendel law as a single recessive gene.Based on the maps of morphological and molecular marker,SSR,RFLP and COS markers were selected corresponding to ps-2 locus.In total,5 SSR markers,13 CAPS markers proved developed from RFLP probe sequences and one COS marker were screened.The linkage analysis that one SSR marker (SSR450) and one CAPS marker (TG123) were tightly linked with ps-2 gene.The linkage distances were about 4.9 and 11.6 cM respectively and both presented co-dominant character.Markers obtained can be directly used for marker assisted selection and the identification of hybrid purity.It also would speed up the procedure for the introgression and application of tomato male sterility in breeding.