2010•Acta Agriculturae ZhejiangensisRequires access
Identification of AFLP markers linked to TYLCVD-resistance gene in tomato.
Yao JinXiao, Yuejian Yang, Qingjing Ye, Rongqing Wang, Meiying Ruan, Xinmei Hou, Zhuping Yao
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Abstract
F1 and F2 progeny of cross combination with TYLCVD-resistant tomato material T0727 and susceptible material T9179 were inoculated.With 64 EcoRⅠ/MseⅠ primer combinations,AFLP analysis was performed on two parents,their F1 and F2 resistant and susceptible bulks.A total of about 4 023 distinguishable bands were amplified,three of which were stable.Genetic linkage analysis of the three polymorphic DNA fragments with the resistance genes was tested in the F2 segregating population derived from the cross with T0727 and T9179.The DNA fragment E-ACC/M-CAG was found to be closely linked to one of the TYLCVD-resistant genes,with a genetic distance of 9.5 cM,which was tentatively named Afty-196.The cloned fragment E-ACC/M-CAG was sequenced and then successfully converted to a SCAR marker,which can be used in marker-assisted selection for tomato resistant to tomato yellow leaf curl virus disease.