2008•Unpublished venueRequires access

Simultaneous Determination of the Contents of Tetrahydropalmatine and Protopine in Rhizoma Corydalis by RP-HPLC

Zeping Li

Abstract

A reserved-phase HPLC method for simultaneous determination of the contents of tetrahydropalmatine and protopine in Rhizoma Corydalis is built up.By using a Phenomenex Luna C18 column with mobile phase methanol-phosphate buffer(pH7.65,65∶35) and detected at wavelength of 281 nm,the average recoveries respectively for tetrahydropalmatine and protopine are 101.77 % and 97.93 %,RSD are 1.34 % and 1.18 %.

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Key concepts: Tetrahydropalmatine, Protopine, Corydalis, Chromatography, High-performance liquid chromatography, Chemistry, Alkaloid, Medicine