2007•ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSISRequires access

A Primary Study on Reducing Browning of Callus Induced from Aloe saponaria Haw Callus

Xiaolu Zhou

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Abstract

The study on the reducing browning of callus induced from Aloe saponaria Haw indicated that: pretreatment before inoculation was useful for reducing callus browning.Soaking explants in pvp(2 g/L)15 min showed distinct effect;darkness culture reduced browning greatly,and benefited callus's growth;media with addition of antioxidant or sorbent showed different effects,antioxidant was better than sorbent.On the basis of fresh callus weight acquiring,the browning restraining ability was as follows: L-Glutamine(5 mg/L)AgNO3(15 mg/L) Vc(2 mg/L) PVP(0.5 mg/L) Citric acid(5 mg/L) Na2S2O3(10 mg/L) blank Ac(10 mg/L).

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What this paper is about

The study on the reducing browning of callus induced from Aloe saponaria Haw indicated that: pretreatment before inoculation was useful for reducing callus browning.Soaking explants in pvp(2 g/L)15 min showed distinct effect;darkness culture reduced browning greatly,and benefited callus's growth;media with addition of antioxidant or sorbent showed different effects,antioxidant was better than sorbent.On the basis of fresh callus weight acquiring,the browning restraining ability was as follows: L-Glutamine(5 mg/L)AgNO3(15 mg/L) Vc(2 mg/L) PVP(0.5 mg/L) Citric acid(5 mg/L) Na2S2O3(10 mg/L) blank Ac(10 mg/L).

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Available abstract

The study on the reducing browning of callus induced from Aloe saponaria Haw indicated that: pretreatment before inoculation was useful for reducing callus browning.Soaking explants in pvp(2 g/L)15 min showed distinct effect;darkness culture reduced browning greatly,and benefited callus's growth;media with addition of antioxidant or sorbent showed different effects,antioxidant was better than sorbent.On the basis of fresh callus weight acquiring,the browning restraining ability was as follows: L-Glutamine(5 mg/L)AgNO3(15 mg/L) Vc(2 mg/L) PVP(0.5 mg/L) Citric acid(5 mg/L) Na2S2O3(10 mg/L) blank Ac(10 mg/L).

Key concepts: Browning, Callus, Citric acid, Inoculation, Chemistry, Explant culture, Horticulture, Darkness

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