Cyclamen Callus Induction Research on Anti-browning

The experimental used Cyclamen (Cyclamen persicum Mill) France Murray Mi F2 plants as the materials, selected the same plant leaf, petiole, and tubers as explants to callus induction,to chose the best medium callus.Callus for induction in the process of browning were discussed from different aspects, found the best browning control of the medium and culture conditions,results showed that: the best callus medium was MS+6-BA(0.5 mg/L)+2,4-D(2 mg/L)+KT(0.2 mg/L)+agar(9 g/L)+sugar(30 g/L);best browning control training callus-induction in the medium to add Vc(2 mg/L)or PVP(0.5 mg/L);dark to a certain extent, curb the occurrence of browning; Although the activated carbon could inhibit browning but also inhibit Cyclamen callus growth.