2005•Zhongguo yaofangRequires access

Content Determination of Luteolin in Wild Chrysanthemum Flower by HPLC

Qian Pin-fei

Abstract

OBJECTIVE:To establish a HPLC method for content determination of luteolin wild chrysanthemum flower.METHODS:The separation was performed on a Kromasil C18 column,the mobile phase was composed of tetrahydrofuran-methanol-1.5%acetic acid(27∶4∶69),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 353nm,the column temperature was 30℃.RESULTS:The calibration curve showed good linearity in the range of 0.028μg～0.56μg(r=1.0 000),the average recovery was 99.9%(RSD=2.28%).CONCLUSION:The method is simple,convenient and accurate and can be used for the quality control of wild chrysanthemum flower.

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Key concepts: Chromatography, Calibration curve, High-performance liquid chromatography, Acetic acid, Luteolin, Content determination, Chemistry, Methanol