Studies on the Callus Induction and Browning Inhibition of Camptotheca acumianta

[Objective] The research aimed to screen out the optimum induction medium for Camptotheca acumianta explants and study the methods of inhibiting browning of explants and callus in subculture.[Method] With the young leaves and seed embryos as explants,through determining POD and PPO activities,the study on callus induction and inhibiting the browning of explants and callus in subculture was conducted from aspects such as explant selection,medium kinds,hormone matching and treatment of adding matte.[Result] The optimal induction medium was SH+NAA 2.5 mg/L+2,4-D 0.5 mg/L+6-BA 0.25 mg/L for the explants of young leaves and MS+2,4-D 1.0 mg/L +6-BA 0.5 mg/L for the explants of seed embryos.The way to inhibit the browning of young leaf explants was to enhance explants sizes and dry the water on the surface of medium.The way to inhibit the browning of callus in subculture was to supplement 30 mg/L Vc and 5g/L AC into the optimal induction medium or to transfer the compact callus to MS medium after the first subculture.[Conclusion] This study provides theoretcal base for genetic transformation and plant regeneration of C.acumianta.