Study on Enzymatic Extraction process of Polysaccharide from GANODERMA and Its Oxidation Resistance

[Objective] To obtain the optimal enzymatic extraction process of polysaccharide from GANODERMA and discuss its oxidation resistance in vitro.[Method]Using extraction rate of polysaccharide as index,the proportion of three enzymes and enzymolysis conditions were optimized by orthogonal test based on single factor test.The in vitro oxidation resistance of polysaccharide was determined by DPPH free radical model.[Result] Complex enzyme extraction was superior to single enzyme extraction,and the optimal use levels of complex enzymes were: cellulose of 1.5%,benase of 0.8% and bromelain of 3.5%(mass fraction,relative to concentration of zymolyte).The optimum enzymolysis conditions were as following: pH value of 5.5,temperature of 50 ℃ and enzymolysis duration of 100 min.In comparison with GANODERMA polysaccharide extracted by water,that extracted using complex enzyme was stronger in DPPH radical-scavenging ability(P0.01),and the DPPH radical-scavenging rate assumed a rise trend along with the increase of polysaccharide concentration in the range of 0.8-4.8 mg/ml.[Conclusion] The study provides scientific basis for the development of GANODERMA polysaccharide.