Enzymatic extraction and DPPH free radical scavenging properties of polysaccharides from Ganoderma lucidum.

[Objective] This study was to investigate the optimal process for extraction of polysaccharides from Ganoderma lucidum via mulienzyme complex,so as to explore their in vitro anti-oxidation capacity.[Method] Using polysaccharides extraction rate as indicator,we performed an orthogonal experiment based on single factor test to optimize enzymolysis combination and enzymolysis conditions,employed free radical DPPH model to assess the in vitro anti-oxidation capacity of G.lucidum polysaccharides.[Result] Enzymolysis extraction was superior to single enzyme extraction.The optimized parameters for the extraction of G.lucidum polysaccharides via enzymmolysis were as follows:cellulose of 1.5%,papain of 0.8% and bromelin of 3.5%(mass fraction,relative to concentration of zymolyte),pH value of 5.5,temperature of 50 ℃ and enzymolysis duration of 100 min.G.lucidum polysaccharides extracted via enzymmolysis performed good capacity to scavenge free radical DPPH,which is significantly higher than that extracted via hydrolysis(P0.01).Within concentration range of 0.8-4.8 mg/ml,G.lucidum polysaccharides assumed a boosting trend in the DPPH-scavenging capacity with the increase of polysaccharides concentration.[Conclusion] Our results provided scientific basis for the exploitation of G.lucidum polysaccharides.