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2008•Anhui Medical and Pharmaceutical JournalRequires access

Determination of baicalin in sanhuang tablet by improved HPLC

Yu Jin

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Abstract

Aim To determine baicalin in sanhuang tablet by improved HPLC.Methods HPLC was used,and the methanol-0.1%trifluoroacetic acid(45∶55) was served as mobile phase,and detection wavelength was 280 nm.Results The linearity range of baicalin was with in the range of 24.0～120 mg·L-1(r=0.999 1,n=5).The average recovery rate was 98.6%(RSD=1.3%,n=5).Conclusion This method is stable,easy,accurate,and can be used in quality control of sanhuang tablet.

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What this paper is about

Aim To determine baicalin in sanhuang tablet by improved HPLC.Methods HPLC was used,and the methanol-0.1%trifluoroacetic acid(45∶55) was served as mobile phase,and detection wavelength was 280 nm.Results The linearity range of baicalin was with in the range of 24.0～120 mg·L-1(r=0.999 1,n=5).The average recovery rate was 98.6%(RSD=1.3%,n=5).Conclusion This method is stable,easy,accurate,and can be used in quality control of sanhuang tablet.

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Available abstract

Aim To determine baicalin in sanhuang tablet by improved HPLC.Methods HPLC was used,and the methanol-0.1%trifluoroacetic acid(45∶55) was served as mobile phase,and detection wavelength was 280 nm.Results The linearity range of baicalin was with in the range of 24.0～120 mg·L-1(r=0.999 1,n=5).The average recovery rate was 98.6%(RSD=1.3%,n=5).Conclusion This method is stable,easy,accurate,and can be used in quality control of sanhuang tablet.

Key concepts: Baicalin, High-performance liquid chromatography, Chromatography, Chemistry, Trifluoroacetic acid

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