Effect of protoplast regeneration technology on the viruselimination of Pleurotus ostreatus

Three dsRNA bands with sizes of 8.2kb,2.6kb and 1.1kb respectively,were isolated from two Pleurotus ostreatus strains,Xin-831 and Yu-6. Three virus-eliminated methods,i.e. hyphal tips isolation virus-elimination (HTIVE),primordium tissue isolation virus-elimination (PTIVE) and protoplast regeneration virus-elimination (PRVE) were applied to prepare virus-eliminated strains,and the effect of virus-elimination was detected by dsRNA technique. The results showed that no dsRNA remained in the strains by PRVE method. However,3 dsRNA bands still remained by PTIVE method and one dsRNA band of 2.6kb,remained by HTIVE method. The results also showed that the hyphal growth rate,biomass,breath intensity and cellulose activity of virus-free strains by PRVE method were obviously superior to the original strains and strains by HTIVE and PTIVE methods. In addition,the biological efficiency of virus-free strains was 96.4% to 99.1%,and 19.9%-25.4% higher than the original strains. Moreover,the length and width of pileus increased. In all,these results indicated that protoplast regeneration technique could effectively eliminate virus of the strains and improve yield of P. ostreatus.