Studies on Virus-free Strain of Pleurotus ostreatus

Pleurotus ostreatus was one of the major cultivated edible fungi in China. However,Pleurotus ostreatus virus can seriously influenced the yield and quality of P. ostreatus. dsRNA was widely existed in P. ostreatus,detected by the dsRNA technique. Some strains with different detoxification extent were obtained by detoxicating four generations continuously with the hyphae tip of virus-free method. dsRNA segments obviously weakened or reduced by dsRNA detection. It suggested that the amount of virus and genome reduced. The average growth rate of virus-free strain improved 28. 1%. The activities of carboxy methyl cellulose enzyme and laccase improved 33. 3% and 20. 6% respectively. The production of fruiting increased 13. 6%,and also the activities of anti-pollution was higher than that before.