The effect of compound 861 on hepatic stellate cell proliferation and collagen synthesis

Objective To observe the effects of compound 861 (Cpd 861) on hepatic stellate cell line (HSC - T6) proliferation, intracellular calcium concentration and collagen synthesis caused by Angll . Methods Cell proliferation of HSC - T6 was detected with Methyl - 3H thymidine (3H - TdR) incorporation. Intracellular calcium, loaded with Fluo - 3 acetoxymethyl ester, was measured with laser scanning confocal microscope in HSC - T6. Collagen synthesis and gene expression in HSC - T6 were detected by enzyme linked immunosorbent assay (ELISA) and RT- PCR. Results AngII could cause an increase in intracellular calcium concentration and cell proliferation. All these effects were blocked by Cpd 861. Cpd 861 downregulated the elevation of Col I and Col III mRNA levels in HSC - T6, and decreased Co I and PIII P levels in culture supernants of HSC - T6. Conclusion Cpd 861 can decrease cell proliferation, intracellular calcium concentration and cell collagen synthesis via AT1R.