Angiotensin II stimulates the synthesis of nucleic acid, protein and collagen in cultured hepatic stellate cells in rats

Objective To investigate the effects of different doses of angiotensin Ⅱ(Ang Ⅱ) on hepa tic stellate cells(HSC) proliferation and collagen synthesis in rats. Methods The pronase E and collagen Ⅰ were used to isolate HSC, 3H TdR, 3H Leu and 3H Pro incorporation methods were used to evaluate the effects of different doses of AngⅡ on HSC DNA, protein and proline synthesis. Results It showed that 10 -9 ～10 -6 mol/L AngⅡ could significantly increase the 3H TdR incorporation rate of HSC ( P 0.05), but only 10 -6 mol/L and 10 -7 mol/L AngⅡ could significantly increase the 3H Leu incorporation rate of HSC ( P 0.01); 10 -9 ～10 -6 mol/L AngⅡ could significantly increase the 3H Pro incorporation rate of HSC in a dose dependent style. Conclusions The appropriate dose of AngⅡ may promote HSC in proliferation and collagen synthesis.