Experimental Study of XiaoChaiHu Decoction Combined with Cyclophosphamide on Tumor Interstitial in Rats of EAC Liver Cancer Mice

[Objective] Discuss the anti-cancer function and mechanism of small entrapement soup and western chemotherapeutics cyclophosphamide on EAC mice affected liver cancer.[Methods] 40 mice from Kuming,hypodermic injection of 2.0×106/mL EAC cancer liver mice cell line 0.2mL/,treat it with entrapement soup,cyclophosphamide,entrapement soup and cyclophosphamide separately,Immune group test the law endothelium growth factor express.The morphological changes were observed under the light microscope.Then find the situation of hepatumor cells with transmission electron microscope.[Results] Immunohistochemistry showed,XiaoChaiHu group,CTX group,XiaoChaiHu +CTX group VEGF po-sitive expression,XiaoChaiHu group,CTX group,XiaoChaiHu +CTX group VEGF po-sitive expression rate and average strength in dyeing were lower than the control group,XiaoChaiHu +CTX group to reduce the most obvious.Under light microscope,XiaoChaiHu group,CTX group,XiaoChaiHu +CTX group can reduce the quantity of tumor stromal capillaries in newborn,XiaoChaiHu +CTX group is the most obvious.XiaoChaiHu group is the second.Under electron microscope,XiaoChaiHu group,CTX group,XiaoChaiHu +CTX group can damage the structure of tumor stromal capillaries in newborn,xcht+CTX group to damage the most obvious.[Conclusions] XiaoChaiHu Decoction combined with Cyclophosphamide may reduce liver cancer in mice EAC tumor VEGF protein expression and restrain the grown of the Tumor interstitial vascularity.