Assay of Nucleosides and Nucleotides in Hippocampus by HPLC

In order to establish a method for the determination of nucleosides( cytidine,uridine,inosine,guanosine and adenosine) and nucleotides( CMP,UMP,IMP,GMP and AMP) in hippocampus,the simultaneous determination was performed on an Accalaim C30( 2. 1 mm × 150 mm,3 μm) column by HPLC. Gradient elution was performed with water( A),100 mmol / L NH4 OAC solution( adjusted p H to 5. 0)( B)- acetonitrile( C) as the mobile phase. The flow rate was 0. 4 m L / min,the system was performed at 25 ℃ and the nucleosides and nucleotides were monitored and quantified at a wavelength of 254 nm. The five nucleosides and the five nucleotides showed good linearity with their concentrations in the range of 0. 02 ~ 10 mg / L. Their average recoveries were between 88. 38% and 96. 30%. The HPLC method established in this study is a rapid and simple method for the simultaneous determination of nucleosides and nucleotides in Hippocampus and is helpful to its quality control.