HPLC Determination of Three Components of Bidens bipinnata L. Extract

OBJECTIVE To establish a HPLC method for determination of bipinnatapolyacetyloside,hyperoside and isoquercitrin in Bidens bipinnata L.extract.METHODS The HPLC method was performed on a Agilent ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm) with MeOH-acetonitrile-0.4% phosphoric acid(24∶6∶70) as the mobile phase.The flow rate was 1.0 mL·min-1.The detection wavelength was 255 nm,and the column temperature was set at 25 ℃.RESULTS The calibration curves of bipinnatapolyacetyloside,hyperoside and isoquercitrin were linear in the ranges of 0.002 587-0.258 7 μg(r=0.999 8),0.009 944-0.497 2 μg(r=0.999 8),0.010 13-0.506 7 μg(r=0.999 7),respectively.The average recoveries(n=6) of the three components from the samples were 97.86%,101.11% and 102.71%,respectively.CONCLUSION This method is accurate,simple and reliable to determine the contents of bipinnatapolyacetyloside,hyperoside and isoquercitrin in Bidens bipinnata L.extract.