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Determination of hyperoside in Pyrola callitha extraction by HPLC

Peng Yin

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Abstract

Objective To establish a method for the determination of hyperoside in Pyrola callitha extraction. Methods The analysis was performed by HPLC with C18 column.The mobile phase consisted of methanol∶0.2% phosphoric acid(45∶55)(pH3.0 adjusted by triethylamine)with detection wavelength at 360 nm. Results The linear range was 15.6-78.0 μg/ml for the hyperoside(r=0.999 9).The average recovery and relative standard deviation were 99.88% and 1.14%(n=9),respectively. Conclusion The method is accurate and feasible for the determination of hyperoside in Pyrola callitha H.Andres extraction.

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What this paper is about

Objective To establish a method for the determination of hyperoside in Pyrola callitha extraction. Methods The analysis was performed by HPLC with C18 column.The mobile phase consisted of methanol∶0.2% phosphoric acid(45∶55)(pH3.0 adjusted by triethylamine)with detection wavelength at 360 nm. Results The linear range was 15.6-78.0 μg/ml for the hyperoside(r=0.999 9).The average recovery and relative standard deviation were 99.88% and 1.14%(n=9),respectively. Conclusion The method is accurate and feasible for the determination of hyperoside in Pyrola callitha H.Andres extraction.

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Available abstract

Objective To establish a method for the determination of hyperoside in Pyrola callitha extraction. Methods The analysis was performed by HPLC with C18 column.The mobile phase consisted of methanol∶0.2% phosphoric acid(45∶55)(pH3.0 adjusted by triethylamine)with detection wavelength at 360 nm. Results The linear range was 15.6-78.0 μg/ml for the hyperoside(r=0.999 9).The average recovery and relative standard deviation were 99.88% and 1.14%(n=9),respectively. Conclusion The method is accurate and feasible for the determination of hyperoside in Pyrola callitha H.Andres extraction.

Key concepts: Hyperoside, Phosphoric acid, Chromatography, High-performance liquid chromatography, Extraction (chemistry), Triethylamine, Chemistry, Biochemistry

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