Clinical observation on disinfection efficacy of Benzalkonium Chloride to the spinal canal puncture skin

Objective To investigate the safety and efficacy on local skin sterilization at punctured region by using Benzalkonium Chloride before vertebral canal puncture.Methods Thirty-five hospitalized patients who were scheduled for spinal canal anesthesia surgery were observed,local skin at punctured region was disinfected with 0.1% of Benzalkonium Chloride before manipulation,10 to 15 cm emittance around the central point.Sterilized 2 times,each specimens were obtained with aseptic cotton bud at a distance of 5 cm far from the point,just at upper,bellow,left and right of the point when the local skin to be natural dry,and inoculated on a sanguineous agar medium immediately cultured for 24 hours in a homeothermia incubator,and then the colony growth situation medium was taken out and colony growth was observed.Results There was no pathogenic bacteria found in one hundred and forty points gathered from 35 patients altogether after 24 hours homoeothermic cultured,only a little micrococcus was found in 1/4 specimen from one patient,the rate of sterilization with 0.10% Benzalkonium Chloride was about 99.29%,and the disinfection effects was satisfaction.Conclusion Satisfactory sterilization effects can be obtained on local skin sterilization at punctured region by using Benzalkonium Chloride before vertebral canal puncture with 0.10% of Benzalkonium Chloride,no remarkable skin stimulation and irritability,the effect is safe and reliable.