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Synthesis and Identification of Artificial Antigen for the Atrazine

Danni Fu

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Abstract

The hapten of atrazine,which was synthesized by using technical grade atrazine reacted with β-Mercaptopropionic acid in the hot alkalic solution and its structure was confirmed by IR,UV and NMR.The hapten was successfully conjugated to bovine serum albumin(BSA) with the active ester method to form artificial immune antigen.According to accumulation principle of UV absorption,the conjugated-ratios of BSA and OVA are 16:1 and 6:1 by compared UV absorptions of hapten,carrier proteins and artificial antigen.

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What this paper is about

The hapten of atrazine,which was synthesized by using technical grade atrazine reacted with β-Mercaptopropionic acid in the hot alkalic solution and its structure was confirmed by IR,UV and NMR.The hapten was successfully conjugated to bovine serum albumin(BSA) with the active ester method to form artificial immune antigen.According to accumulation principle of UV absorption,the conjugated-ratios of BSA and OVA are 16:1 and 6:1 by compared UV absorptions of hapten,carrier proteins and artificial antigen.

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Available abstract

The hapten of atrazine,which was synthesized by using technical grade atrazine reacted with β-Mercaptopropionic acid in the hot alkalic solution and its structure was confirmed by IR,UV and NMR.The hapten was successfully conjugated to bovine serum albumin(BSA) with the active ester method to form artificial immune antigen.According to accumulation principle of UV absorption,the conjugated-ratios of BSA and OVA are 16:1 and 6:1 by compared UV absorptions of hapten,carrier proteins and artificial antigen.

Key concepts: Hapten, Atrazine, Bovine serum albumin, Conjugated system, Antigen, Chemistry, Absorption (acoustics), Chromatography

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