Protective Effect of Yixinyin on Myocardial Ischemia-Reperfusion Injuries

Aim This study aims to observe the protective effect of Yixinyin on myocardial ischemia-reperfusion injuries in isolated working hearts of tats.Method 36 rats were randomly divided into three groups:Yixinyin group 1 (K-H fluid contain 0.01g·ml~(-1) Yixinyin ),Yixinyin group 2 (K-H fluid contain 0.1g·ml~(-1) Yixinyin) and control group.Myocardial founction was investigated by left ventricular systolic pressure (LVSP),rising and descending rate of left ventricular pressure (±dp/dt(max)),left ventricular end diastolic pressure (LVEDP),heart rate;tissue levels of malondialdehyde(MDA)as well as activity of super oxide demitasse(SOD) were measured in order to evaluate the changes of oxygen free radical metabolism; electron microscope and light microscope were performed to investigate the impairment of myocardial structure and Ca~(2+) distribution.Results Myocardial dynamics and homodynamic indexes LVSP and ±dp/dt(max) were improved siganificantly in Yixinyin group2;the content of MDA in Yixinyin group 2 was obviously lower than that in control group(P0.05). Yixinyin reduced abnormal of Myocardial structure and Ca~(2+) distribution by comparison with other groups.Conclusion Yixinyin have protective effects against the injury of myocardial ischemia-reperfusion.The effects increase when concentration of Yixinyin increased.