STUDIES ON THE GENETIC VARIATION OF ONCOMELANIA HUPENSIS WITH SSR-PCR

Objective To detect genetic variation of different isolates of Oncomelania hupensis in China. Methods The simple sequence repeat anchored PCR (SSR PCR) technique was performed to amplify genomic DNA. The genetic distance was measured and dendrogram was constructed. Results The SSR PCR products were polymorphic. The genetic distance between the Chinese Taiwan isolate and the Chinese mainland isolates of O. hupensis was 0.351±0.048(0.263-0.445); The genetic distance between the Chinese mainland isolates of O. hupensis from hilly regions, Yunnan Eryuan and Sichuan Tianquan, was 0.043; The genetic distance among the Chinese mainland isolates of O. hupensis from lake regions was 0.000-0.217; The genetic distance between the Chinese mainland isolates of O. hupensis from hilly regions and that from lake regions was 0.182-0.308; The genetic distance among the Chinese mainland isolates of O. hupensis from the same province, Hubei Province, was 0.000-0.167. Conclusion The Chinese mainland strain of O. hupensis can be divided into four groups in different level: ①Yunnan Eryuan and Sichuan Tianquan;②Anhui Guichi, Hunan Yueyang, Hubei Jingmen and Jiangxi Xinjian;③Hubei Wuhan, Hubei Zhongxiang, Hubei Yangxin, Hubei Songzi, Hubei Shashi, Hubei Shishou and Hubei Yingcheng;④Hubei Chibi. In addition, the Chinese Taiwan isolate can be divided a different group.