Genetic variation of Schistosoma japonicum with SSR-PCR

Aim To detect genetic variation of different isolates of Schistosoma japonicum.Method The simple sequence repeat anchored PCR(SSR PCR)technique was performed to amplify genomic DNA.The genetic distance was measured and dendrogram was constructed.Results The SSR PCR products were polymorphic.The genetic distance among the Taiwan isolate and the Chinese mainland isolates of S.japonicum was 0 316±0 067(0 176～0 378);the genetic distance between the Chinese mainland isolates of S.japonicum from hilly regions,Yunan Eryuan and Sichuan Tianquan,was 0 067;the genetic distance among the Chinese mainland isolates of S.japonicum from lake regions was 0 063～0 176;the genetic distance among the Chinese mainland isolates of S.japonicum from hilly regions and that from lake regions was 0 067～0 250;the genetic distance among the Chinese mainland isolates of S.japonicum from the same province,Hubei province,was 0 063～0 111 Conclusion Chinese mainland strain of S.japnicum can be divided into five groups in different level;①Yunnan Eryuan and Sichuan Tianquan;②Anhui Guichi and Hunan Yueyang;③Hubei Wuhan and Hubei Zhongxiang;④Jiangxi Xinjian and Hubei Yangxin;⑤Hubei Songzi.In addition,the Taiwan isolate can be divided into a different group.