Protective effect and its mechanism of Curcumin on ischemia reperfusion injury of skeletal muscle

Objective: To observe the effect of plasma contents of creatinephosphokinase(CPK),lactatedehpdrogesase(LDH),malondialdehy(MDA) and the absorbed dose of 99mTc methylene diphosphonate(99mTcMDP) in skeletal muscle,investigate the protection and mechanism of Curcumin on skeletal muscle ischemia reperfusion injury.Methods: Ischemic reperfusion model of the hindlimb was established,and 30 rats were randomly divided into three groups such as sham group,ischemia reperfusion control and Curcumin group.The degree of muscle damage was measured by changes of MDA,CPK,LDH in plasma contents and the absorbed dose of 99mTcMDP in skeletal muscle,and the microstructure of gastrocnemius muscle were observed in every group.Results: The plasma contents of CPK,LDH,MDA and the absorbed dose of 99mTcMDP in skeletal muscle in control(7 296.18±1 086.53,1 203.66±282.53,10.36±2.65,16.69±3.14) and Curcumin group(5 168.49±975.39,726.56±203.65,6.78±2.12,11.45±2.35) were significantly higher than those in sham group(3 014.26±963.78,463.85±75.32,3.54±1.89,9.12±1.96).While the plasma contents of CPK,LDH,MDA and the absorbed dose of 99mTcMDP in skeletal muscle in Curcumin group were significantly lower than those in control group(P 0.01),and the microstructure of gastrocnemius muscle in Curcumin group was significantly improved compared with control group.Conclusion: Curcumin could decrease the plasma contents of CPK,LDH,MDA and the absorbed dose of 99mTcMDP in skeletal muscle,and relieve the degree and limit of necrosis on skeletal muscle ischemia reperfusion injury,and improve the microstructure of skeletal muscle in ischemia reperfusion injury.So Curcumin has a protection on the skeletal muscle ischemia reperfusion injury.