Effects of curcumol on proliferation and apoptosis in human cervical cancer CASKI cell line

Objective:To investigate the effects of curcumol on proliferation and apoptosis in human cervical cancer CASKI cell line.Methods: CASKI cells cultured in vitro were separately treated with curcumol at concentration of 12.5,25,50 and 100μg/ml.The cell proliferation was evaluated by Methyl thiazolyl tetrazolium (MTT) assay.Cell cycle distribution and apoptosis were detected by flow cytometry (FCM).Cell morphology was studied by transmission electron microscope.Results: When CASKI cells were treated with 12.5,25,50 and 100μg/ml curcumol,the proliferation of CASKI cells was apparently inhibited in a dose- and time-dependent manner.When CASKI cells were treated with curcumol at 50 and 100μg/ml for 24 h,the CASKI cells were arrested in G2/M phase and showed typical apoptosis characteristics in FCM and transmission electron microscope analysis.Conclusion: Curcumol can inhibit the proliferation of CASKI cells,block cell cycle at the G2/M phase,and induce cell apoptosis.